Isotopes | High Resolution Mass Spectrometry

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IsotopesSince a mass spectrometer separates and detects ions of slightly different masses, it easily distinguishes different isotopes of a given element. This is manifested most dramatically for compounds containing bromine and chlorine, as illustrated by the following examples. Since molecules of bromine have only two atoms, the spectrum on the left will come as a surprise if a single atomic mass of 80 amu is assumed for Br. The five peaks in this spectrum demonstrate clearly that natural bromine...

HOW TO CLEAN pH ELECTRODES | HOW TO REJUVENATE pH ELECTRODES

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HOW TO CLEAN pH ELECTRODESCleaning of the electrode (note that in case of gel electrodes replacing of the reference solution is usually impossible):GeneralSoak in 0.1M HCl for half an hour.Drain and refill the reference solution.Soak the electrode in filling solution for one hour.Inorganic:Soak in 0.1M tetrasodium EDTA solution for 15 minutes.Drain and refill the reference solution.Soak the electrode in filling solution for one hour.Protein:Soak in 1% pepsin / 0.1M HCl for 15 minutes.Drain and refill the reference solution.Soak the electrode in filling solution for one hour.Grease and Oil:Rinse with detergent or ethanol solution.Drain and refill the reference solution.Soak the electrode in filling solution for one hour. Electrode response...

Bond Stretching

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Energy due to Bond Stretching Whenever a bond is compressed or stretched the the energy goes up. The energy potential for bond stretching and compressing is described by an equation similar to Hooke's law for a spring, except a cubic term is added. This cubic term helps to keep the energy from rising too sharply as the bond is stretched....
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